Spain head coach Luis Enrique has brought Álvaro Morata and Koke back into the national squad, while Marcos Llorente has received his first senior call-up.
Morata and Koke back in after lengthy absences from Spain team
La Roja have been short of in-form central strikers in recent months but Morata has enjoyed a strong start to the season with Juventus, scoring six goals in seven appearances in all competitions, including three in his past two games.
Morata's last appearance for Spain was in a 5-0 Euro 2020 qualifying win over Romania in November 2019.
Atlético Madrid midfielder Koke has also returned, having last played for his country in a 4-1 friendly defeat of Wales more than two years ago.
Marcos Llorente rewarded for Atlético performances
Team-mate Marcos Llorente has earned his first call-up, while Liverpool's Thiago Alcántara is out due to problems with a knee injury and Arsenal's Dani Ceballos has been omitted.
With Diego Llorente injured, Íñigo Martínez returns, as does Fabián Ruiz. Rodrigo Moreno is left out after testing positive for coronavirus this week.
⚠️ ¡TENEMOS NUEVA CONVOCATORIA! ⚠️
— Selección Española de Fútbol (@SeFutbol) November 6, 2020
🤔 ¿Hay alguien por aquí que no haya jugado alguna vez al fútbol chapas?
🏟️ @LUISENRIQUE21 anuncia su lista de 2⃣5⃣ jugadores para los compromisos ante Países Bajos, Suiza y Alemania en un estadio muy especial.#SomosFutbol#SomosFederación pic.twitter.com/1d7gYU1MDN
Chelsea goalkeeper Kepa Arrizabalaga keeps his place despite having lost his starting spot under Frank Lampard to new signing Edouard Mendy.
Spain to take on Netherlands, Switzerland and Germany
Spain face the Netherlands in a friendly on Wednesday, before Nations League clashes with Switzerland and Germany.
Spain squad:
Kepa Arrizabalaga (Chelsea), David de Gea (Manchester United), Unai Simón (Athletic Bilbao); Sergi Roberto (Barcelona), Jesús Navas (Sevilla), Sergio Ramos (Real Madrid), Eric García (Manchester City), Pau Torres (Villarreal), Íñigo Martínez (Athletic Bilbao), José Gaya (Valencia), Sergio Reguilón (Tottenham); Rodri (Manchester City), Sergio Busquets (Barcelona), Fabián Ruiz (Napoli), Sergio Canales (Real Betis), Mikel Merino (Real Sociedad), Koke (Atlético Madrid), Marcos Llorente (Atlético Madrid); Adama Traoré (Wolves), Dani Olmo (RB Leipzig), Ferran Torres (Manchester City), Gerard Moreno (Villarreal), Álvaro Morata (Juventus), Ansu Fati (Barcelona), Mikel Oyarzabal (Real Sociedad).